Tomentosin shows anticancer effect on U87 human glioblastoma multiforme cells

Glioblastoma multiforme (GBM) is one of the most common brain tumors. Chemotherapy, radiotherapy and surgical resection are methods used in GBM treatment, however, investigation possible anticancer effects low-toxicity natural products on various cancer cells, including GBM, leads to promising results. In this study, it was aimed investigate effect tomentosin, which a sesquiterpene lactone, U87 human cells. The cytotoxic tomentosin evaluated by XTT assay. concentration that inhibits 50% cell viability (IC50) determined results from XTT, and, further analyzes cells were treated with at IC50 concentration. Then, total RNA isolation cDNA synthesis performed control dose groups, evaluating expression levels important genes associated apoptosis metastasis qPCR analysis. addition, colony forming capacity formation According our results, found be 28.8 µM for 48 hours. When compared group, increased BAX, CASP3, CASP8, CASP9, CYCS, FADD, TNF, TNFR1, TNFR2 TIMP2 genes. And, tomantosin significantly decreased conclusion, thought exerts its changing